Part:BBa_K562008:Design

Salty_PduABTUN BMC Components

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1898
  Illegal BamHI site found at 2490
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1441
  Illegal AgeI site found at 1426
  Illegal AgeI site found at 2040
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Native PstI sites have been removed from pduB and pduU. There is an engineered BamHI site at the extreme 3'end.

Source

Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence.